高效液相色谱法测定人血清中的苄氟噻嗪、环戊噻嗪
Guangzhou Chemical Industry(2013)
Abstract
以乙醇-K2HPO4-水双水相体系进行样品前处理结合高效液相色谱法分别提取测定了人血清中苄氟噻嗪和环戊噻嗪。试验采用80%(V∶V)乙醇水溶液为萃取液,然后加入适量K2HPO4形成双水相,样品中的亲水性基质保留在双水相下相,而苄氟噻嗪和环戊噻嗪等目标分析物质进入双水相上相,实现了药物残留的高效、快速萃取。双水相上相可直接供HPLC法测定。本方法测定苄氟噻嗪和环戊噻嗪的检出限分别为0.0087μg·mL-1与0.0089μg·mL-1。加标回收率均大于93%。
MoreTranslated text
Key words
alcohol,HPLC method,bendroflumethiazide,human serum albumin,cyclopenthiazide,aqueous two-phase systems
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined