Monitoring Proteins Using In Vivo Near-Infrared Time-Domain Optical Imaging after 2- O -Hexyldiglycerol-Mediated Transfer to the Brain
Molecular Imaging and Biology(2010)
摘要
Purpose The aim of the present study was to gain insight into the penetration, biodistribution, and fate of globulins in the brain after 2- O -hexyldiglycerol-induced blood–brain barrier opening. Procedures The spatial distribution of fluorescence probes was investigated after blood–brain barrier opening with intracarotid 2- O -hexyldiglycerol injection. Fluorescence intensity was visualized by microscopy (mice and rats) and by in vivo time-domain optical imaging. Results There was an increased 2- O -hexyldiglycerol-mediated transfer of fluorescence-labeled globulins into the ipsilateral hemisphere. Sequential in vivo measurements revealed that the increase in protein concentration lasted at least 96 h after administration. Ex vivo detection of tissue fluorescence confirmed the results obtained in vivo . Conclusion Globulins enter the healthy brain in conjunction with 2- O -hexyldiglycerol. Sequential in vivo near-infrared fluorescence measurements enable the visualization of the spatial distribution of antibodies in the brain of living small animals.
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关键词
BBB opening,2-O-Hexyldiglycerol,Anti TGF-beta antibody 1D11,Alexa Fluor 680,Cy5.5,Time-domain optical imaging
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