响应面法优化去内毒素乳铁蛋白纯化工艺的研究
China Dairy Industry(2013)
Abstract
通过采用离子交换色谱法来去除乳铁蛋白中的内毒素,通过鲎试剂来检测内毒素的含量,并使用响应面法优化了乳铁蛋白的纯化工艺。结果表明:去内毒素后乳铁蛋白中内毒素从原来的18.26 EU/mL降低为0.77 EU/mL,在洗脱剂pH值为4.83,洗脱速度1.21 bV/h,洗脱剂浓度0.05 mol/L醋酸钠缓冲溶液条件下,蛋白得率可达到最大值62.87%。
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Key words
remove endotoxin,lactoferrin,ion-exchange,response surface methodology
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