Process of Pollen Sprouting and Pollen Tube Growth on the Lily Distant Hybridization

This article studied the process of pollen sprouting and pollen tube growth on the lily distant hybridization combination 'Bernini'×'Pollyanna' by the fluorescence microscope.We observed that pollen sprouting formed the pollen tube after pollination in 3~30 h.The speed of pollen tube growth from quick to slow,then stopped growing between 48~51 h,finally penetrated into 1/3 of style.Besides,we also observed some unusual pollen tube shapes.During the pollen tube growth,it was companied with a series of callose responses,which was happened in the pollen tube,papilla,canal cell,embryo sac in ovule.The results indicated that this hybrid combination was incompatible.We suggested that the 'Bernini' papilla of stigma is the first hindrance which preventing the 'Pollyanna' pollen tube from growing and the canal cell is the second barrier that preventing the pollen tube from growing in stylar canal.