HPLC测定舒阴洗液中苦参碱的含量
Chinese Journal of Experimental Traditional Medical Formulae(2010)
Abstract
目的:建立舒阴洗液中苦参碱的含量测定方法.方法:采用高效液相色谱法,Inertsil ODS-SP色谱柱(4.6 mm×150 mm,5 μm),流动相乙腈-水-三乙胺(3∶97∶0.2,用磷酸调pH 3.5),流速1.0 mL·min-1,检测波长220 nm.结果:苦参碱在3.9~117.0μg呈良好的线性关系(r=0.999 9),平均回收率为101.69%,RSD 1.84%(n=9).结论:本法简便、快速、准确,可用于该制剂的质量控制.
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Key words
HPLC,Shuyin lotion,matrine
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