RGD多肽表面修饰生物陶瓷修复骨缺损BMP-2的表达
Chinese Journal of Aesthetic Medicine(2007)
Abstract
目的:了解精氨酸-甘氨酸-天冬氨酸(Arg-Gly-Asp,RGD)多肽表面修饰的羟基磷灰石-磷酸三钙(hydroxyapatite-tricalcium phosphate,HA-TCP)修复节段性骨缺损局部骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)的表达。方法:以骨髓基质干细胞(marrow stromal cells,MSCs)复合RGD多肽表面修饰的HA-TCP或单纯材料培养制备组织工程骨,选择60只新西兰白兔,制作15mm长的桡骨节段性骨缺损模型,实验根据植入不同的材料分为A、B、C和D组,A组:骨缺损区植入MSCs复合RGD多肽表面修饰的HA-TCP培养制备的组织工程骨;B组:骨缺损区植入MSCs复合HA-TCP培养制备的组织工程骨;C组:骨缺损区植入RGD多肽表面修饰的HA-TCP;D组:骨缺损区植入HA-TCP。术后4周取材,行修复区局部BMP-2免疫组化分析。结果:术后4周各组骨缺损区均有新骨生成,修复区局部BMP-2表达水平依次为:A>B>C>D(P<0.001,P<0.05)。结论:RGD多肽表面修饰对以HA-TCP为支架材料组织工程骨的修复作用有明显优化作用。
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Key words
tissue engineering,surface modification,marrow stromal cells,bone morphogenetic protein
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