亚硒酸钠诱导K562细胞凋亡作用及机制探讨
Journal of Chongqing Medical University(2007)
Abstract
目的:探讨亚硒酸钠诱导K562细胞凋亡的作用及机制.方法:不同浓度亚硒酸钠作用K562细胞后,MTT法检测细胞增殖抑制,电镜和TUNEL法检测K562细胞的凋亡,RT-PCR检测Bcl-2和Bax的mRNA表达变化,分光光度法检测Caspase-3的活性变化.结果:亚硒酸钠能抑制K562细胞的生长增殖,诱导其凋亡.20μmol/L亚硒酸钠作用K562细胞48h后,Bcl-2表达明显降低,Bax表达明显升高,Caspase-3活性升高,与对照组相比具有显著的统计学意义(P<0.01).结论:亚硒酸钠能够诱导K562细胞凋亡,其机制可能与下调Bcl-2,上调Bax,活化Caspase-3有关.
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Key words
Sodium selenite,Apoptosis,K562
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