高效液相色谱法测定人血浆中麦考酚酸的含量
Journal of Medical Science Yanbian University(2012)
Abstract
[目的]建立人血浆中麦考酚酸的高效液相色谱定量方法.[方法]色谱柱为安捷伦C18(150.0mm×4.6mm,5μm)色谱柱,流动相为0.05mol/L磷酸钠(pH值为2.88)-乙腈(65∶35),流动速度为1.0mL/min,柱温为30℃,检测波长为240nm,以卡马西平为内标物.[结果]麦考酚酸在0.5~50.0mg/L血药质量浓度范围内时血药质量浓度与峰面积比值呈良好的线性关系(r=0.999 7);低、中、高质量浓度的提取回收率分别为89.77%,99.43%,100.39%,方法回收率分别为102.33%,104.62%,107.39%;日内、日间精密度RSD均小于7%,样品在室温24h内、-20℃冰冻及冻融等条件下稳定性良好.[结论]利用高效液相色谱法测定麦考酚酸的血药质量浓度具有简便、快速、准确、重现性好等特点,可用于临床服用吗替麦考酚酸酯后麦考酚酸血药质量浓度监测及其药物代谢动力学研究.
MoreTranslated text
Key words
chromatography,mycophenolate mofetil,mycophenolic acid,plasma concentration,high pressure liquid
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined