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怀地黄试管苗脱毒技术研究

LI Ming-jun,ZHANG Xiao-li,DU Lin,ZHAO Xi-ting, LIU Jie,ZHOU Na,TU Rong-tao,GUO Dan

Journal of Henan Normal University(Natural Science)（2008）

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Abstract
对怀地黄试管苗的脱毒技术进行了研究.结果发现:以MS为基本培养基并附加适宜浓度细胞分裂素(6-BA0.5 mg.L-1或KT0.5 mg.L-1)时,茎尖成苗率最高(75%),出愈率较低(20%);切离的茎尖大小为0.2~0.5 mm时,既能保证较高的脱毒率又可保证较高的成苗率;两次切离茎尖并与热处理相结合,试管苗的脱毒率最高(96%~100%),建立了一种有效的怀地黄脱毒方法和技术.
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Key words
Plant regeneration rate,Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao,Heat treatment,Shoot tip culture,virus-free percentage
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