肺炎支原体的分离鉴定与药敏性研究
Chinese Journal of Clinical Medicine（2012）
Abstract
目的:探讨肺炎支原体的临床分离株对临床常用药的敏感性.方法:将临床采集的咽试子标本经过选择性培养基筛选培养,同时进行聚合酶链反应(polymerase chain reaction,PCR)检测,确定分离到的支原体为肺炎支原体.将肺炎支原体与不同浓度的临床常用抗生素混合,观察液体培养基颜色的变化,判断肺炎支原体对药物的敏感性.结果:成功分离到1株肺炎支原体；阿奇霉素、红霉素和头孢呋辛钠浓度分别稀释至31μg/mL、125μg/mL、500μg/mL时,肺炎支原体抑制；而头孢曲松钠、美洛西林、氨苄青霉素等抗生素对支原体无抑制作用,培养基颜色均由红色变为橙黄色.结论:肺炎支原体临床分离株对阿奇霉素高度敏感,对红霉素和头孢呋辛钠中度敏感.
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Key words
Drug sensitivity,Mycoplsma pneumoniae,Isolation and identification
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