Multiple Chromatograms Identification and Quality Control of Clematis filamentosa from Different Producing Areas

OBJECTIVE:To establish the TLC,HPLC,FTIR and GC-MS fingerprints of Clematis filamentosa for quality control of it.METHODS:HPLC method was used to determine the content of kaempferide.The determination was performed on Waters Xbridge C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-0.4% acetic acid(gradient elution).The detection wavelength was set at 326 nm;Developer of ligroin-methanol(9:1) and chloroform-methanol(9:1)were used in ligroin extract and acetic ether extract respectively in TLC.Absorption peaks were labeled in petroleum ether and ethyl acetate by FTIR.The volatile oil components were analyzed by GC-MS.RESULTS:The common characteristic peaks in TLC,HPLC and IR fingerprints of C.filamentosa were used for qualitative analysis;the content of kaempferide was measured by HPLC;37 components were identified by GC-MS.CONCLUSION:Multiple chromatograms can be used for comprehensive identification and quality control of C.filamentosa.