Detection of Exogenous Genes in Genetically Modified Plants with Multiplex Polymerase Chain Reaction
Plant molecular biology reporter(2001)
Abstract
To detect exogenous genes in genetically modified plants, we have designed a multiplex polymerase chain reaction (MPCR) system that includes 7 primer pairs. With this method, the promoter, terminator, selection gene, reporter gene, and function gene can be detected simultaneously. The concentrations of each primer were optimized according to AT/TA%. Several genes in plasmid PBI121, genetically modified tobacco, and imported soybeans were detected, and the results were consistent with standard PCR. The method was sensitive enough to detect a single copy sequence.
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Key words
genetically modified plants,multiplex PCR,PBI121,soybean,tobacco
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