Multiple genes code for high-molecular-mass rhoptry proteins of Plasmodium yoelii
Molecular and Biochemical Parasitology(1995)
Abstract
We have examined the number of genes coding for a group of high-molecular-mass rhoptry protein(s) in the malaria parasite Plasmodium yoelii, and studied variation in the gene family within the parasite's genome. A region of the genes was amplified using oligonucleotides based on conserved DNA sequences and the products cloned. The sequences could be divided into 7 groups by restriction-fragment-length polymorphism. Further variation was detected by sequence analysis; 11 different sequences were detected in the 16 clones analyzed. The genes in the family were distributed on 6 chromosomes probably at 9 or more loci.
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Key words
Rhoptry protein,Multigene family,Plasmodium yoelii,Malaria
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