乙醇提取红豆杉树叶紫杉醇醇提条件的研究

Biotechnology Bulletin(2013)

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Abstract
对南方红豆杉树叶中紫杉醇的最佳醇提工艺条件进行了研究。采用乙醇浸提方法,考查了粉碎度、乙醇浓度、料液比、提取温度和提取时间对紫杉醇提取效率的影响,经过一系列的单因素试验和正交试验,得到了紫杉醇醇提工艺的最佳条件:在粉碎度120目、95%乙醇浸提、料液比为1∶15、提取温度40℃、提取4 h、重复2次的条件下,经乙酸乙酯—丙酮(6∶4)洗脱纯化,HPLC测定紫杉醇含量,可使紫杉醇含量达到0.012 5 mg/g。
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Taxus chinensisvar.maireiPaclitaxel Ethanol extraction HPLC
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