[Effects of CD74 gene on IFNγR gene expression in MG TEC].

AIM:To study the effects of CD74 gene on IFN-γR expression in myasthenia gravis thymic epithelial cell(TEC). METHODS:PCMV-CD74 transiently transfect to primary cultured TEC mediated with lipofectamine, the result of transfection and some autoimmune related genes such as IFN-γR were detected by real-time RT-PCR. RESULTS:Real-time quantitative RT-PCR results show that the mRNA expression level of IL-4R, IL-32, IFN-γR, ECGF1, HLA-A, HLA-DR increased significantly, with the high express ion of CD74 gene in thymic epithelial cells. CONCLUSION:CD74 gene over-expression in TEC can increase IFN-γR mRNA expression.