Rat liver 6-phosphogluconolactonase: A low Km enzyme

By specific enzymic synthesis of the substrate 6-phosphogluconolactone in situ , the K m for rat liver 6-phosphogluconolactonase was found to be 80 μM. This value is approximately 100 fold lower than the previously determined value, and is compatible with the kinetic paramaters of both glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, and hence with the flux through the oxidative segment of the pentose phosphate pathway.