Antagonistic activity and mechanism of Bacillus subtilis CG-6 suppression of root rot and growth promotion in Alfalfa

Root rot is a common disease, that severely affects the yield and quality of alfalfa. Biocontrol is widely used to control plant diseases caused by pathogenic fungi, however, biocontrol strains for alfalfa root rot are very limited. In this study, a Bacillus subtilis CG -6 strain with a significant biocontrol effect on alfalfa root rot was isolated. CG -6 secretes antibacterial enzymes and siderophore, phosphate solubilization and indoleacetic acid (IAA). The inhibition rate of strain CG -6 against Fusarium oxysporum was 87.33%, and it showed broad-spectrum antifungal activity. Inoculation with CG -6 significantly reduced the incidence of alfalfa root rot, the control effect of greenhouse cultivation reached 58.12%, and CG -6 treatment significantly increased alfalfa plant height, root length, fresh weight, and dry weight. The treatment with CG -6 significantly increased the levels of antioxidant enzymes (catalase, peroxidase, superoxide dismutase, and lipoxygenase) in alfalfa leaves by 15.52% - 34.03%. Defensive enzymes (chitinase and beta -1,3-glucanase) increased by 24.37% and 28.08%, respectively. The expression levels of regulatory enzyme genes ( MsCAT , MsPOD , MsCu, Zn-SOD1 , MsCu, Zn-SOD2 , MsCu, Zn-SOD3 , and MsLOX2 ) and systemic resistance genes ( MsPR1 , MsPDF1.2 , and MsVSP2 ) increased by 0.50 - 2.85 fold, which were higher than those in the pathogen treatment group. Therefore, CG -6 could be used as a potential strain to develop biopesticides against alfalfa root rot.