SNPs of DECR1 gene and its genetic polymorphism in pigs: SNPs of DECR1 gene and its genetic polymorphism in pigs

Three hundred and twenty seven Yorkshire,Landrace and Duroc pigs were analysed to screen the SNPs in 6-10 Exon of mitochondrial 2,4-dienoyl-CoA reductase 1(DECR1) gene and analyse its genetic structure.The resluts showed: the primers were designed accroding to human DECR1 gene by means of genome comparison,2 SNPs were screened out in Exon6 and Exon8 by PCR-SSCP method,but mutation in Exon6 loic belonged to nonsense mutation,mutation in Exon8 loci belong to missense mutation.The polymorphism of the SNP in Exon8 were studied and 3 genotypes(CC,CG,GG) were tested in 3 breeds.There were abundant genetic polymorphism in Exon8 loci.