苏云金杆菌及其微胶囊剂对落叶松毛虫的毒力及抗紫外线能力
Forest Pest and Disease(2010)
Abstract
从呼和浩特市大窑林场自然死亡的落叶松毛虫Dendrolimus superans幼虫体分离Bacillus thuringiensis菌株Bt-S04,通过形态观察和生理生化反应并与菌株Bt-k01进行对照,Bt-S04其精氨酸酶、尿酶和水杨苷反应均为阴性,菌膜反应和蔗糖发酵反应均为阳性,与苏云金杆菌松毛虫变种有差异.室内采用针叶给毒法,测定了Bt-S04和Bt-k01菌株对落叶松毛虫2龄幼虫的毒力,其致死中浓度LC50分别为5.493×106(1.349×106~1.698×107)CFU/mL和7.227×105(3.111×105~1.399×106)CFU/mL,Bt-k01对落叶松毛虫2龄幼虫的毒力是Bt-S04的7.6倍,致死中时间是其1/5.经紫外光照射2 h后,Bt-k01和Bt-S04活菌率分别是5.4%和8.2%,微胶囊化后菌液成活率分别是72%和76.3%,表明微胶囊剂的抗紫外线能力增强了.
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Key words
microcapsule,toxicity,Dendrolimus superans,Bacillus thuringiensis
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