Protective Role of CGMP in Intestinal Mucosal Barrier Function in Rats

Objective: To explore the effect of caseino glycomacropeptide(CGMP) intervention on the expression of trefoil factor and vascular endothelial growth factor in the colon and deduce the effect of CGMP on key factors of intestinal mucosal barrier function in rats with colorectal cancer.Methods: A total of 40 Wistar rats were randomly divided into five groups including control group,model group,low-dose CGMP group,middle-dose CGMP group and high-dose CGMP group.Except the control group,all the rats were intraperitoneally injected with dimethyl hydrazine at a dose of 30 mg/kg.At the same time,the rats from CGMP groups were injected with 1,2-dimethylhydrazine dihydrochloride and administered with CGMP at dosages of 10,50,100 mg/(kg.d),respectively.After eight weeks,all the rats were killed to harvest colon tissues for determination of the expression of ITF and VEGF by immunohistochemical method.Results: HE staining revealed that CGMP might regulate damaged intestinal barrier function in rats.Meanwhile,IHC staining confirmed that CGMP could reduce the expression of VEGF,but had no effect on the expression of TFF3.Conclusion: CGMP may repair damaged intestinal barrier function in rats.