[Establishment of a multiplex PCR detection method for four foodborne pathogens].

OBJECTIVE:To establish a multiplex PCR method for simultaneous detection of Salmonella, Shigella, Staphylococcus aureus and Listeria monocytogenes in food. METHOD:The primers were designed according to the sequences of invA gene of Salmonella, ipaH gene of Shigella, nuc gene of Staphylococcus aureus and hly gene of Listeria monocytogenes, and the reaction system was optimized. RESULTS:No false positive and false negative results occurred during the test of 15 target strains and 17 non-target strains of bacteria, and the molecular weight of PCR products were consistent with expected. The detection limits for each pathogen were at least 1 pg/microl. The sequence analysis of PCR products showed the same sequences with target genes. The test result of 319 samples showed that 4 raw milk samples were contaminated by staphylococcus aureus, and 1 raw pork sample by Salmonella. CONCLUSION:The established method showed good specificity, and provided an important method for the rapid detection of foodborne pathogens.