Cytotoxicity and acute toxicity study on vinblastine (VLB) nanoparticles.

【Objective】 The aim of this study was to investigate toxicity of VLB poly(buty1 cyanoacrylate) nanoparticles(PBCA-NP).【Method】 Difference between VLB-PBCA-NP and crude VLB on the growth of L929 and rat astrocyte was measured by MTT assay and morphology method.In addition,after the same dose of VLB-PBCA-NP and crude VLB solution were injected into mice tail vein,50% lethal dose of(LD50) was counted.【Results】 The results indicated that there was no significant difference on the effect of rat astrocyte growth between 0.5-50 ng/mL VLB-PBCA-NP and crude VLB solution,but 500 and 5 000 ng/mL VLB-PBCA-NP significantly degraded cytotoxicity(P0.05).5 000 ng/mL VLB-PBCA-NP belonged to toxicity grade 3 on L929 fibroblasts growth,and very significantly degraded crude VLB toxicity.The LD50 of VLB-PBCA-NP and crude VLB solution was respectively 26.38 and 22.7 mg/kg.The LD50 of VLB-PBCA-NP increased by 16.21% compared with crude VLB solution.【Conclusion】 VLB-PBCA-NP degraded acute toxicity and nontumorous cells toxicity of crude VLB.