In Planta Agroinfection By Canadian And German Potato Leafroll Virus Full-Length Cdnas

Full-length infectious cDNA clones of Canadian (PLRVflc) and German (PLRVflc) Potato leafroll virus isolates were constructed and their biological activities examined in planta. For both constructs, agroinfection by PLRV cDNA resulted in virus Multiplication in the primary leaf followed by systemic movement and the production of icosohedral PLRV particles. Differences between the two constructs Occurred with respect to infection, movement, and titres of infectious virus produced from the two full-length cDNAs. This study reports the first PLRV agroinfection and comparison of biological characteristics between two independent infectious Luteoviridae cDNA clones.