Automation for Cryo-TEM: From Specimen Grid to 3D Map

neral biological community as well as in increasing the volumeof data that can be collected during a single session at the microscope.The Leginon system that we have developed1-2is designed to emulate all of the decisions and actions of ahighly trained microscopist in collecting data from a vitreous ice specimen. These include identifyingsuitable areas of vitreous ice at low magnification, determining the presence and location of specimen on thegrid, automatically adjusting imaging...