重组人胰岛新生相关蛋白的高水平表达与纯化
China Biotechnology（2007）
Abstract
通过RT-PCR体外扩增目的基因胰岛新生相关蛋白(islet neogenesis associated protein,INGAP),并将其克隆入原核表达载体pET22b(+),在大肠杆菌BL21(DE3)中诱导表达.表达的目标蛋白主要以包涵体形式存在,洗涤杂蛋白后用尿素溶解,经Heparin Agrose亲合柱层析分离后,再用Superdex75凝胶过滤层析进一步纯化.纯化后的INGAP皮下注射免疫家兔,制备兔抗INGAP血清,采用免疫双扩、ELISA评价INGAP的免疫活性.结果显示INGAP表达量高达菌体总蛋白的40%左右,经HPLC测定,分离纯化后的目标蛋白纯度达到98.81%,且具有良好的免疫原性.
MoreTranslated text
Key words
Islet neogenesis associated protein Expression Purification Immunologenicity
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined