低强度超声对鼻咽癌CNE2细胞增值和凋亡的影响
Laser Journal(2009)
Abstract
目的:观察低强度超声对人鼻咽癌CNE2细胞增殖和凋亡的影响。方法:以低强度超声(频率1.7MHz,剂量1.35W/cm2)辐照CNE2细胞,采用MTT观察超声对COC1细胞增殖的抑制作用;光镜观察细胞形态,细胞核荧光染色法检测凋亡细胞。结果:超声波能够显著抑制鼻咽癌CNE2细胞增殖,辐照后孵育18h,其MTT光吸收值与对照组相比显著降低:光镜下可见细胞形态学改变,同时Hoechst33258荧光染色法观察到亮蓝色着染的凋亡细胞。结论:低强度超声辐照对人鼻咽癌CNE2细胞体外生长具有明显的抑制作用,并可诱导其凋亡。
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Key words
CNE2 cell,apoptosis,low intensity ultrasound
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