Microvessel count(MV),tumor associated macrophage and mast cell count in pancreatic cancer and non-cancerous pancreatic tissues of SD rats

Objective To investigate relation of the MV, count of tumor associated macrophage (TAM) and mast cell (MC) in pancreatic cancer tissues and non-cancerous tissues of rats.Methods Immunohistochemical method of ABC was used for detecting the MV TAM, MC counts on the routinely paraffin-embedded sections of 17 cases of pancreatic cancer tissues of rats, 22 cases of non-cancerous pancreatic tissues of rats and 10 cases of normal pancreatic tissues of rats.Results In 36 rats, 17 cases of pancreatic ductal adenocarcinoma(47.2 %) within 3-5 months in 36 rats with implanted DMBA. The mean cunt of MV, TAM and MC in the 17 cases of pancreatic adenocarcinoma tissues of rats was 76.56±19.93, 15.54±5.93 and 14.87±5.19 respectively, which was significantly higher than that in the 10 cases of normal pancreatic tissues (30.63±14.77, 11.01±4.99 and 11.04±5.03), which was also higher than those in the model group of non-cancerous pancreatic tissues (24.42±11.32,10.99±4.9 and 9.31±4.64). The counts of TAM and MC of rats of pancreatic cancer within five months were higher than that within 3-4 months (P0.01). The closely positive correlations were also found among the counts of MV, TAM and MC(MV vs TAM, r=0.788; MV vs MC, r=0.832; TAM vs MC, r=0.635).Conclusions A high incidence of pancreatic cancer can be obtained in a short time by use of DMBA directly implanted into the parenchyma of pancreas in rats. TAM and MC might have important effect on angiogenesis of pancreatic cancer of SD rats.