Cloning and sequences analysis of the complement C3d cDNA of porcine, mouse and sheep.
Chinese Journal of Preventive Veterinary Medicine(2009)
摘要
The C3d gene from mouse,porcine and sheep were amplified from total RNA by RT-PCR using primes designed according to C3d gene sequences of human,mouse and cattle.The cDNA fragments were cloned and sequenced.Bioinformatics analyses showed that the C3d of mouse had a sequence similarity of 83 %,82 %and 81 % at the amino acid level with human,porcine and sheep C3d respectively,and the C3d of sheep shared 94 % similarity with cattle.The complement receptor2(CR2)-binding site on C3d of mouse,porcine and sheep was highly conserved,suggesting these receptor binding regions have consistent structure and function.
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关键词
clone,complement c3d,sequence analysis,complementary dna,binding sites,rna,reverse transcriptase pcr,dna cloning,genes
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