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Release Of Nucleotide-Cleaving Acid-Phosphatase From Carrot Cells Grown In Suspension-Culture

G. Ciarrocchi,R. Cella,E. Nielsen

PHYSIOLOGIA PLANTARUM（1981）

Cited 22|Views2
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Abstract
Carrot (Daucus carota L. cv. Lunga di Amsterdam) cells grown in suspension culture release into the culture medium a phosphatase capable of converting deoxyribo- as well as ribonucleoside triphosphates into nucleosides. The enzyme activity requires acidic pH and allows a prompt utilization of phosphorylated DNA precursors as measured by tritiated dTTP incorporation. Such utilization is partially inhibited by inorganic phosphate and completely inhibited by ATP.
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Key words
nucleotides,acid phosphatase
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