Renal and hepatic glutathione concentrations in rats after treatment with hexachloro-1,3-butadiene and citrinin

Renal and hepatic glutathione (GSH) concentrations were examined after treatment of male Sprague-Dawley rats with hexachloro-1,3-butadiene (HCBD) or citrinin alone and in combination, and after pretreatment with the GSH depleting agent diethylmaleate (DEM). It was found that both renal and hepatic GSH depletion were greater when either citrinin or HCBD was given following DEM. The effect was particularly striking when the doses used were so low as to be ineffective when given alone. When HCBD and citrinin were given in combination, the effect on GSH was approximately additive. Renal tubular organic ion transport in kidney slices was also compromised significantly when either citrinin or HCBD followed pretreatment with DEM. With HCBD, depression of tetraethylammonium (TEA) transport was seen after DEM; when given alone HCBD had no effect on TEA transport.