Effect of Hypoxic Training on the Numbers of CD34~+ Cells and CD34~+/Flk-1~+ Cells in Rats' Peripheral Blood

Objective: To study the effect of hypoxia,training and hypoxic training on the numbers of CD34+ cells and CD34+/Flk-1+ cells in rats' peripheral blood.Materials and Methods: 60 male SD rats are randomly divided into six groups.Progressive treadmill exercise and hypoxia are used to establish hypoxic training animal model.Peripheral blood lymphocytes of rats are separated by Ficoll-Urografin density gradient centrifugation.Flow cytometry is applied to exam phenotype of peripheral blood mononuclear cells.The results show that the percentage of peripheral blood CD34+ cells are 0.19%,0.28%,0.75%,2.04%,6.51%,8.39% in normoxic control group,hypoxic living group,normoxic training group,normoxic living hypoxic training group,hypoxic living normoxic training group,and hypoxic living hypoxic training group respectively.Hypoxic living normoxic training and hypoxic living hypoxic training increase the percentage of peripheral blood CD34+ cells significantly,compared with normoxic control group.The percentage of peripheral blood CD34+/Flk-1+ cells are 0.03%,0.10%,0.26%,0.65%,1.65%,2.42% in normoxic control group,hypoxic living group,normoxic training group,normoxic living hypoxic training,hypoxic living normoxic training,and hypoxic living hypoxic training respectively.Normoxic living hypoxic training,hypoxic living normoxic training,and hypoxic living hypoxic training increase the percentage of peripheral blood CD34+/Flk-1+ cell significantly compared with normoxic control group.Conclusion: Together with hypoxic living and hypoxic training can obtain high CD34+ cell and CD34+/Flk-1+ cell mobilization rates,and CD34+ cell mobilization rate is higher than that of CD34+/Flk-1+ cell.In addition,CD34+/Flk-1+ cell may take part in angiogenesis in cardiac muscle as an cell offer.