Blood compatibility and mechanical properties of oxidized-chitosan films

By dipping chitosan films into saturated NO2-glacial acetic acid solution, the hydromethyl groups on the film surface could be oxidized to carboxyl groups and the blood compatibility of the films improved. Fourier transform infrared (FTIR) spectra indicated the presence of many -COOH and -COO- groups on the modified membrane surface. Scanning electron microscopy (SEM) showed that the surface of the modified membrane was rough, as compared with the chitosan film, which possessed a smooth surface. In the oxidation process, with increased dipping time of the films in saturated NO2-glacial acetic acid solution, the tensile strength of the films decreased slowly initially, and rapidly 10 h later. The swelling ratio of the modified chitosan film increased obvious noticeably as the degree of oxidation of the film increased. All antithrombosis and hemolysis tests and blood cell morphology observation with SEM revealed that the blood compatibility of modified chitosan membranes is superior to that of chitosan films. (c) 2007 Wiley Periodicals, Inc.