Screening of SCAR Marker Linked to Mink Self-biting Disease

RAPD was used to analyze genetic constitution of the healthy and self-biting mink groups,and was converted into SCAR marker.Five polymorphic primers were screened from 100 random primers by RAPD.Specific and non-specific amplified DNA fragments,HA-400 and SA-500,were found,cloned and sequenced.Based on these sequences,two specific primers of SCAR were designed(SHA-400 and SSA-500).Then PCR amplification was carried out for the healthy and self-biting minks.Results showed that almost all of mink were amplified by SHA-400 and SSA-500.The frequency of SHA-400 in the two groups was 82.5% and 22.5% respectively,and χ2 test of polymorphic fragments in the two groups showed significantly difference(P<0.001).The frequency of SSA-500 in the two groups was 87.5% and 97.5% respectively,and no significant difference was observed between the two groups(P>0.05).It is indicated that the polymorphic fragment(HA-400)can be used as a positive marker candidate.