Activation of LA-N-2 cell phospholipases by single alanine substitution analogs of amyloid β peptide (25–35)

A series of single alanine substituted analogs of amyloid β peptide (25–35) were tested for their ability to activate the phospholipases of cultured LA-N-2 cells. Substitution of alanine for the amino acids 29–34 prevented the activation of phospholipases A2 and D. In addition substitution of alanine at 28 prevented phospholipase D but not phospholipase A2 activation. All the alanine substitutions, except for positions 33 and 35, blunted phospholipase C activations. There were no activations by scrambled amyloid β peptide.