Analysis of HLA active specific immunotherapy for 98 cases of infertility before IVF-ET

Objective To assess the validity of HLA active specific immunotherapy for infertility.Methods This study was conducted in 98 URSA patients who were given intradermal injection of their husbands' leukomonocyte once two weeks.The injection sites were divided into 6~8 areas.Then they came to the secondary therapy course after six weeks.One or two more therapy courses should be used in these patients who got negative results of HLA-DR antibody testing and pregnant patients.Results 32 patients of primary infertility and 15 patients of secondary infertility were HLA-DR antibody positive after one course of HLA active therapy,and the effective rate was82% and 88% respectively.15 patients of primary infertility and 11 patients of secondary infertility got the positive results of HLA-DR antibody after two courses of HLA active therapy,and the effective rate was 79% and 85%.HLA-DR antibody of 32 patients of primary infertility and 2 patients of secondary infertility changed to positive after three courses of HLA active therapy,and the effective rate was 43% and 67% respectively.45 patients were pregnant after HLA active immunotherapy,and the achievement ratio was 46%.Conclusion HLA active specific immunotherapy may increase the pregnancy rate of infertility patients with negative HLA-DR antibody.HLA-DR antibody can be a useful marker for the diagnosis and immunotherapy of infertility.