Analysis of annual drug resistance of bacteria isolated from body fluid specimens between 2006 and 2008 for Military Antimicrobial Resistance Surveillance.

Objective To summarize annual distribution of microbial population and drug resistance of bacteria isolated from body fluid specimens for Military Antimicrobial Resistance Surveillance(MARS)in Xi'an region between 2006 and 2008.Methods Isolated bacteria were cultured by routine method and identified by automatic bacteria analysis system or ATB system.Drug susceptibility test was done according to CLSI standards.Data statistical analysis was conducted by means of WHONET5.4 software.Results In two years,493 strains were isolated from body fluid specimens and the ranked order from first to fifth was Escherichia coli,Klebsiella pneumoniae,Staphylococcus aureus,Acinetobacter baumannii and Enterococcus faecius.The detection rate of methicillin resistant staphylococcus aureus(MRSA)was 40.74% while that of methicillin resistant coagulase negative staphylococcus(MRCNS)was 75.00%.The detection rate of Escherichia coli and Klebsiella pneumoniae producing extended -spectrum β -lactamases(ESBL)was 63.33% and 65.91%,respectively.No staphylococcus and enterococcus resistant to vancomycin or linezolid were found.Acinetobacter baumannii and Pseudomonas aeruginosa were all sensitive to Polymyxin B,and their drug resistant rate against other antimicrobial agents commonly used was between 21.70% and 92.00%.Conclusion Escherichia coli is a main pathogen in infected body fluid specimens.Carbapenems,piperacillin/tazobactam,cefoperazone/sulbactam and amikacin show high antibacterial activity against Escherichia coli.However,drug resistance of Acinetobacter baumannii and Enterococcus faecius is a serious problem which we should be paid close attention to.