活血养阴颗粒提取纯化工艺研究
Research and Practice on Chinese Medicines（2006）
Abstract
目的研究活血养阴颗粒制备工艺,优选最佳提取、纯化工艺务件.方法以葛根素含量、含固量为检测指标,用正交试验考察了3种因素(加水量、煎煮时间、煎煮次数)对其水煎煮工艺的影响;然后比较了不同的醇沉浓度的去杂效果.结果活血养阴颗粒最佳水提工艺条件为先加12倍量水,煎煮1.5 h,再加10倍量水,煎煮1 h,煎煮2次;去杂工艺以60%乙醇沉为佳.结论制备工艺合理.
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Key words
HPLC,Huoxue Yangyin granules,puerarin,alcohol precipitation,orthogonal test
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