Dna-Protein Crosslinks Induced By Formaldehyde And Its Repair Process

KCL-SDS assay was used to explore DNA-Protein Crosslinks (DPC) effect induced by formaldehyde (FA) and the repair. Gaseous FA could cause DPC at concentrations as 1.0 mg m(-3) and 3.0 mg m(-3) (p < 0.01), which was removed within 12 h. Meanwhile, in vitro studies showed that 75 mu mol L-1 liquid FA could significantly induce DPC in HcpG2 cells, and was removed after 18 h and 24 h. These results suggested that FA could induce DPC at relatively high concentrations, and removing of DPC in vivo required shorter time than that in vitro.