HPLC法同时检测辣椒制品中8种非食用红色色素
China Condiment(2007)
Abstract
建立了凝胶柱净化-高效液相色谱法同时检测辣椒制品中苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ、对位红、苏丹红G、苏丹红7B和苏丹红B等8种红色色素的方法。样品用正己烷或正己烷/丙酮提取,提取液经BioBeadsS-X3凝胶柱净化。净化后的样品采用ZorbaxSBC18柱分离,以0.1%甲酸水溶液(A)和含0.1%甲酸的甲醇丙酮(9∶1,V/V)溶液(B)为流动相,流速1mL/min,检测波长505nm。上述8种色素组分在其质量浓度为0.16~2.56mg/L时有良好的线性关系,方法的检测限为15~46μg/kg;平均加标回收率为89.9%~118.2%,相对标准偏差为2.3%~4.8%。该方法灵敏可靠,适合于辣椒制品中多种脂溶性红色色素的同时检测。
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Key words
chilli products,gel column,Para red,high performance liquid chromatography,Sudan red
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