日本乙型脑炎病毒SA14-14-2株NS1-Flag蛋白在293T细胞中的表达
China Animal Husbandry & Veterinary Medicine（2011）
Abstract
通过RT-PCR方法扩增出日本乙型脑炎病毒SA14-14-2株的NS1基因,利用HindⅢ和HpaⅠ双酶切克隆到带有Flag标签的pcDNA3.1载体上,重组质粒命名为pcDNA3.1-NS1-Flag。脂质体法转染293T细胞后,经间接免疫荧光(IFA)和化学荧光Western blotting检测,证明带有Flag标签的NS1基因在293T细胞中得到高效表达。
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Key words
eukaryotic expression,Japanese encephalitis virus,293T cell,NS1-Flag protein
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