Cloning and Expression Profiling of Canine Nk1, Nk2 and Nk3 Receptors

l~d:LoA.Mutating 4 or 2 bases through a C-rich element reduced the responses by ->65% and ->40%, respectively.Mutations in other regions of the promoter caused a less marked reduction of the responses, in EMSAs, a nnnrber of complexes bound a wild type (wt) labeled probe, spanning -86 m -64bp, and binding was prevented by inchision at excess unlabeled wt probe.For one complex, mutations of two or more bases through the region -79 to -70 prevented unlabeled probe competing Ibr binding to the complex.Tbe intensity of this complex was not altered by gastrin.De-incuhation of nuclear extract with antibodies to two members of the Sp family of transcription factors, Sp-3 and Sp-4, disrupted binding to the wt probe.Conclusions: 1. Gastrin stimulates Reg expression via activation of RhoA. 2 A C-rich redan (-78 to -71) is critical to the gastrin stimulation of Reg expression 3 Sp family transcription factors bind to this region of the Peg promoter.