Autofluorescence of routinely hematoxylin and eosinstained sections without exogenous markers

Hematoxylin and eosin stained paraffin section was examined by fluorescence microscopy to study the pattern and distribution of fluorescence. Autofluorescence was sensitive and specific for detection of elastic and collagen fibers. It was concluded that analytical morphological techniques based on autofluorescence can obtain information about morphological and pathological state of tissue and cells.