Enterococcus faecalis RQ15产低温中性蛋白酶的纯化及酶学性质
Food Science(2011)
Abstract
采用DEAE Sepharose Fast Flow和SuperdexTM 75对Enterococcus faecalis RQ15产蛋白酶进行纯化和酶学性质研究。SDS-PAGE测定该蛋白酶分子质量为32.4kD,最适作用温度35~40℃,最适pH7.5。20~40℃之间酶活较高,pH值耐受范围广泛,具有低温蛋白酶的特征。Zn2+对蛋白酶有激活作用,Ag+、Hg2+和EDTA-Na2对酶有显著抑制作用。纯酶最适作用条件下对酪蛋白底物的Km和Vmax分别为1.31×10-4mol/L和6.92×10-6mol/(L.s)。
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Key words
Enterococcus faecalis,purification,cold-adapted acid protease
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