Identification and Conjugation of F(ab′)_2 Segment of Human Erythrocyte McAb and Hbs Ab

Non-agglutinating McAb of human RBC was produced.Then,bispecific antibody reagent was prepared for clinical diagnosis.The membrane protein of type O human RBC was used as antigen to immunize the Balb/c mice.Non-agglutinating human RBC McAb was prepared through cell fusion with lymphocyte hybridoma technique.The bispecific antibody reagent is produced through chemical cross-linking of F(abˊ)2 segment of non-agglutinating McAb and HBV surface antibody.The reagent can be used to diagnose HBV surface antigen through direct agglutination reaction.Five non-agglutinating hybridoma cell lines were obtained,and the bispecific antibody was made by one-step glutaraldehyde method.Furthermore,the whole-blood agglutinating test reagent was formed and primary identification experiment was carried out.