Fixation, Staining And Coverslipping Of Frozen Section Slides

This chapter covers fixation, staining and coverslipping of frozen section slides. A number of fixatives and fixative cocktails in use are described and illustrated as well as artifacts resulting from prolonged drying. Several commonly used staining procedures are described with suggestions on maximizing the quality and information gathered for interpretation. Adhesion of tissue to glass slides and causes of poor adhesion is discussed. Problems arising in the coverslipping process are discussed and method for coverslipping finished slides is offered.