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Studies on Rapid Propagation and Plantlet Regeneration of Jerusalem artichoke(Helianthus tuberosus)

Journal of Southwest University(Natural Science Edition)(2013)

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摘要
The effects of MS mediums supplemented with various concentrations of hormones on the proliferation and rooting of Jerusalem artichoke were studied.Its tuber tissue and young stem were used as explants for screening high frequency of adventitious shoot regeneration.The results indicated that the induction rate of adventitious shoot rate from young stem was 100% on MS medium with BA 2mg/L and NAA 0.1mg/L.The propagation coefficient of adventitious shoots was 3.25on the multiplicative culture medium,a 9.6-fold increase per generation.93%of the regenerated adventitious shoots rooted on the rooting medium(MS+ NAA 0.01mg/L).Survival rate of the regenerated plantlets,which were hardened off,was as high as 91.5% when they were transplanted on to the field.
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关键词
Jerusalem artichoke(Helianthus tuberosus),shoot differentiation,propagation,plantlet regeneration
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