茶芪苓方剂有效部位群质量控制的研究
West China Journal of Pharmaceutical Sciences(2013)
Abstract
目的探讨茶芪苓方剂有效部位的质量控制方法。方法采用薄层扫描法测定茶芪苓方剂中黄芪甲苷的含量、紫外分光光度法测定茯苓多糖的含量、HPLC-UV法测定丹参酮ⅡA的含量。结果黄芪甲苷0.2~0.7μg与峰面积线性关系良好,平均回收率为98.37%,RSD=1.70%;茯苓多糖在3.286~13.143μg.mL-1线性良好,平均回收率为99.49%,RSD=0.88%;丹参酮ⅡA0.025~0.200μg与峰面积线性关系良好,平均回收率为98.38%,RSD=2.74%。结论所用方法准确、重复性好,可作为茶芪苓方剂有效部位群的质量控制方法。
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Key words
HPLC,Chaqiling prescriptions,Thin-layer chromatography,UV Spectrophotometry
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