The biological function and mechanism of Myeloma cells on endothelial cells

Objective :To investigate the impact of human multiple myeloma cells U266 on human umbilical vein endothelial cells(HUVEC) proliferation,migration,angiogenesis,and the expression of AKT,pAKT.Methods:Multiple myeloma cells U266 and HUVEC were cultured as a mixed co-culture group to the same period HUVEC cultured alone as the control group to detect the proliferation of HUVEC in the two groups by CCK-8 and the migration of HUVEC and by scratch test to observe HUVEC angiogenesis in Matrigel matrix and to find the expression of AKT and pAKT in HUVEC of co-culture group by Western blot.Results:Compared with the control group,the number of HUVEC cells was markedly increased after co-culture.Migration of endothelial cells in co-culture group was significantly increased compared with that in the control group,the number being 125±21/HP and 332±37/HP.In the Matrigel matrix,the number of tube formation in endothelial cells of the co-culture group was significantly increased compared with that in the control group,the number being 3.7±1.3/HP and 8.3±1.3/HP.The expression of AKT and pAKT in HUVEC was significantly increased after co-cultured,but AKT protein expression was not statistically significant.Conclusion:Multiple myeloma cells can promote the HUVEC proliferation,migration and angiogenesis,showing that the promotion of multiple myeloma cells to HUVEC was related with the AKT signaling pathway.