结核分枝杆菌融合抗原Ag85B-ESAT6真核表达载体的构建及鉴定
Journal of Henan University of Technology(Natural Science Edition)（2006）
Abstract
以结核分枝杆菌H37Rv株的基因组作为模板,将Ag85B和ESAT6编码基因进行PCR扩增,然后采用基因剪接重叠扩增PCR法(gene SOEing)将其通过疏水甘氨酸接头(GGIGIAPG)连接融合,定向克隆至质粒Pvax1中,构建结核分枝杆菌Ag85B-ESAT6融合抗原的真核表达质粒Pvax1/AE.经单双限制性内切酶图谱、PCR产物及DNA测序分析等多种方法鉴定,证实Pvax1/AE真核表达质粒构建成功.为进一步研究其结核核酸疫苗原型的免疫保护效果奠定了基础.
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Key words
tuberculosis,fusion gene,DNA vaccine.,ESAT6,Ag85B
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