Robust and reliable in vitro regeneration of Tossa Jute (Corchorus olitorius L. var. O-9897)

Lack of a rapid and robust in vitro regeneration protocol for Tossa jute (Corchorus olitorius L.) hinders the genetic transformation of jute crops for different agronomic traits of interest. The current study was conducted to develop a rapid, efficient, and reproducibleregeneration protocol for recalcitrant Tossa jute. The potential of auxins (IAA, IBA) and cytokinin (BAP) for the induction of callus, regeneration of shoot and formation of root from the cotyledon with attached petiole of cultivar O-9897 on MS was tested accordingly. Approximately, total 96 calli (out of 100 explants) were induced within 32 days on MS media harboring 2.5 mg/L BAP + 0.5 mg/L IAA. Within 5 weeks, about 95 calli regenerated total 99 shoots (86.6